Fluorometry
The NEONATAL T-GAL Screening Fluo is an enzymatic test for the fluorescence detection of galactose and galactose-1-phosphate in the context of neonatal screening for galactosemia. This quantitative test is performed from a drop of blood dried on blotting paper type 903® and 226.
D-Galactose et Galactose-1-phosphate isolated from dried blood spots react with an enzyme-coenzyme reagent containing alkaline phosphatase (AP), galactose dehydrogenase and NAD+. Galactose-1-phosphate is converted to galactose by AP. The oxidation of galactose by galactose dehydrogenase is driven by the reduction of the coenzyme NAD+ to NADH. The NADH produced catalyzes the action of the diaphorase enzyme on resazurin which is reduced to resorufin. The reaction is measured by fluorescence at a wavelength of 590 nm after an excitation at 544 nm. The signal intensity is directly proportional to the concentration of total galactose present in the sample.
This test is designed to screen newborns for galactosemia using a dried blood sample on blotting paper type 903® or 226.
Product code
The NEONATAL Total Galactose Screening Assay is an enzymatic test used to quantify total galactose (galactose and galactose-1-phosphate) as part of neonatal screening blood test for galactosemia. This test is performed based on dried blood samples taken on 903® and 226 type blotted paper.