Fluorometry
The NEONATAL MSUD Screening Fluo is an enzymatic test which enables the fluorescence detection of 3 amino acids accumulated in case of leucinosis (maple syrup urine disease - MSUD) in newborns. This test allows the quantitative determination of the amino acids leucine, isoleucine and valine from a drop of blood taken on blotting paper type 903® and 226.
Branched-chain amino acids (BCAAs) are extracted from newborn samples. In the presence of a solution containing leucine dehydrogenase and NAD+, the BCAAs are processed and the NAD+ is reduced to NADH. The NADH produced catalyzes the action of the enzyme diaphorase on resazurin which is reduced to resorufin. Excitation of resorufin at a wavelength of 544 nm results in a fluorescence emission at a wavelength of 590 nm whose intensity is directly proportional to the concentration of branched-chain amino acids present in the sample.
This test is designed for neonatal screening for maple syrup urine disease using a dried blood sample on 903® or 226 blotting paper.
Product code
NEONATAL MSUD Screening Assay is a quantitative enzymatic test that assays leukine, one of the 3 amino acids accumulated in maple syrup urine disease (MSUD) also known as ketoacidemia or branched-chain amino acids (BCAAs). This test is for maple syrup urine disease newborn screening, and is performed using dried blood samples on 903® or 226 type blotting paper.