Fluorometry
The NEONATAL PKU Screening Fluo is an enzymatic test designed to screen newborns affected by phenylketonuria. This device allows the fluorescence determination of the quantity of phenylalanine presents in a drop of blood dried on 903® or 226 blotting paper.
Phenylalanine, extracted from neonatal samples, is combined with the enzymatic reagent phenylalanine dehydrogenase. In the presence of NAD+, the enzyme catalyzes the oxidative deamination of phenylalanine to phenylpyruvate and generates NADH. NADH acts as a co-factor for the reduction of resazurin to resorufin by the enzyme diaphorase. The intensity of the resorufin fluorescent signal (emission at 590 nm after excitation at 544 nm) is directly proportional to the concentration of Phenylalanine present in the sample.
This test is designed to screen newborns for phenylketonuria using a blood sample dried on 903® or 226 blotting paper.
Product code
NEONATAL PKU Screening Assay is a quantitative enzyme assay used in phenylketonuria (PKU) newborn screening. It's a PKU test for newborns that quantifies an abnormal concentration of phenylalanine in dried blood samples on 903® or 226 blotting paper.